Acquire your ATM card. Your bank will send your ATM card to you in the mail. Most banks will also send you a separate document that contains a temporary PIN number. Some banks send the PIN number first. When you get the information with your PIN number, your card should arrive within a few days. • Some banks don't send you a PIN number. Instead, they require you to either activate the card and choose a PIN number, or use the 3-digit security code on the back of the card to activate the card before choosing a PIN number. • If you are getting a replacement card, your old PIN number will still work. Verify that the card you received is one you applied for or requested. If you're not expecting an ATM card, contact your bank immediately to find out if the card you received is legitimate. Some banks will issue a new debit card if there was a problem, like potential fraud, associated with your existing debit card. • If you did not request a new debit card, make sure to find out if you need the debit card. Make sure to read the fine print or contact the bank to make sure the debit card doesn't have hidden fees or that switching to a new debit card is required. • Examine the card's expiration date. If the expiration date is approaching soon, contact your bank. This is often a sign of a fraudulent card. Go to an automatic teller machine. Choose an ATM that is well-lit, situated in a visible location, and owned by your banking establishment. Insert your card, use your PIN or your temporary PIN, and follow the instructions on screen. Some banks may require you to go through an activation screen, while other banks may automatically activate the card when you enter the PIN. Others require you to do a balance inquiry. • You may conduct transactions at ATMs that are second- or third-party owned once you activate your card, but they cannot perform the functions necessary to activate your card. ![]() ![]() Report Your Lost or Stolen Debit/ATM Card. If your Pauls Valley National debit or ATM card has been lost, stolen or you find unauthorized transactions on your bank statement, report it immediately. We can deactivate the card and order a replacement card at the same time. Notice: You are leaving the Nicolet National Bank website and the site you are being directed to is not owned or controlled by Nicolet National Bank. ![]()
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Almost every computer and mobile device on the face of the Earth can open a PDF file, which makes the format extremely useful for sharing documents. The person you’re sending the document to doesn’t need any software or fonts to see it exactly what you’re seeing, and thanks to our ever-evolving operating systems, you can now print to PDF in with little to no hassle. Doing so hasn’t always been easy, however. If you’re running an older OS, the option to print PDF may not be available. What’s more, you may want to opt for a third-party alternative, which will afford you additional functionality. Regardless of which method you prefer, though, creating a PDF in Windows is easier now than ever before. Whether you want to use Windows 10’s own PDF printer, or remain loyal to Windows 7 and 8, this guide will walk you through the process every step of the way. Here is our comprehensive rundown of the best ways to print to PDF in Windows. Looking to take things a step further? In order to allow users to save a filled-in PDF with. You must perform this task again to enable Reader users to save their own. A local company that. Feb 18, 2010. This article presents scripts for not only saving a PDF file to disk, but also for saving the PDF to different formats, such as an image file, MS Word, text and. I am new to adobe 11 acrobat pro and have created a form which i intend to have available on a website, enable clients to fill out the form fields and. Mar 12, 2009. When you use Acrobat 8, or 9, select 'enable usage rights' from the Advanced menu. This adds about 20 kb to the pdf. The other possibility is to use CutePDF Pro, add a submit button and have the XFDF data submitted to your self as an email or to a web server. The XFDF data can then reload the original. We also have a guide on how to. How to print to PDF using native tools in Windows 10 MacOS and Linux users have had a PDF printer included by default for more than a decade, and a, but Microsoft didn’t add the feature until Windows 10. Thankfully, it should be activated by default. To use it, go to print your document as you normally would, and select Microsoft Print To PDF as your printer. You’ll be asked where you’d like to save the resulting file, and after a few seconds, you’ll have a brand-new PDF. If for whatever reason Microsoft Print To PDF isn’t listed as an option, don’t fret, as it’s easy to set up. The first step, is to navigate to the Windows 10 Settings page, which you can find in the Start menu. ![]() Once there, click on Devices. The ensuing window automatically opens the Printers & scanners menu, which shows each printer available to the Windows 10 user. This is where — if all was well — you would see Microsoft Print to PDF listed alongside real-world printers. In this scenario, however, it’s not, so to fix that, you need to click Add a printer or scanner at the top of the window. From there, Windows will attempt to find your elusive printer, but since the one we’re trying to install doesn’t exist in the physical world, it will come up short. Simply click the The printer that I want isn’t listed prompt, which should appear shortly. This opens up a new window, one in which you can add a printer manually. Select Add a local printer or network printer with manual settings. In the next window, open the drop-down menu beside Use an existing port, select FILE: (Print to File), and click Next. ![]() ![]() In the following window, choose “Microsoft” as the manufacturer, then scroll down to highlight Microsoft Print To PDF before hitting Next again. When given the choice to replace the current driver or use the driver already installed, we recommend simply using the current driver and selecting Next. After Windows 10 prompts you to name the print-to-PDF feature — we recommend just assigning it the default name so it’s easier to find — click Next one last time to install the desired printer. With the function now available for use, simply open the document or webpage you wish to convert to PDF and navigate to the Print function. When prompted, select Microsoft Print to PDF in the list of printer destinations and click Print to convert the file to PDF. If done correctly, the page will immediately ask you where you’d like to save the PDF document, and to give the file a name. Simply choose a save location on your storage drive, give it a unique name, and hit Save to create your PDF. Once saved, use to make any changes, if need be. ![]() ![]() Are you wondering about “What number to call to activate Verizon Phone?” It sometimes irritates us to activate a brand new phone or the one which is gifted to us. Smartphone dominated environment makes us gauge more, as the contacts, messages, apps and other personal and security details need to be moved too. We bring you the guide to help you in the time of dire need of activating a Verizon cell service. There are multiple ways to activate your Verizon phone but each of it has its own pros and cons. How to Activate Verizon for iPhone • Sync your previous iPhone with iTunes. The contacts and settings will be transferred to your new iPhone. Activate Your Device. Welcome to Boost! You've come to the right place to get your phone up and running. Please make sure you have your phone with you before you start this process. • Call (877) 807-4646 from another phone to activate your 4G LTE plan. This would be unnecessary if your SIM card was already 4G enabled on your previous device. The afore-mentioned number cannot be called from the new iPhone. Follow the prompt after calling and remember to keep your purchase bill accessible. • Your iPhone would already be bundled with a SIM card if you bought it from Verizon, if not insert your own. Then, power up the phone. • Brand new phones take a few minutes to boot up. Slide the “Hello” screen to initiate the setup process. Select the Wi-Fi network if you have a password and the network is secure. • If you cannot find a wireless network, you can plug your iPhone into your computer and use iTunes to activate the device. You will be prompted to activate automatically after plugging the phone in. • Wait for your iPhone to activate. Your iPhone will try to activate without your help as soon as it connects to the wireless network. If you receive a SIM card error, ensure that the correct SIM card is inserted into the iPhone. • Proceed with your iPhone’s default setup. After activating, the setup assistant will continue directing you through the iPhone’s setup process. How to Activate Verizon for Samsung Galaxy/Note • If you are switching from an iPhone, turn the iMessage option off. This ensures that the new messages will come on your new device. Tap Settings, then Messages, go to iMessage and then turn the slider off to do so. • Call (877) 807-4646 from another phone to activate your 4G LTE plan. This would be unnecessary if your SIM card was already 4G enabled on your previous device. The afore-mentioned number cannot be called from the new Samsung phone. Follow the directions received from the prompt after calling and remember to keep your purchase bill accessible. • Check that you have a SIM card installed. If you have just bought the phone, then the battery will not be inserted in it and you can see the SIM slot with the card in it. If there isn’t one, check the packaging. But if you don’t own one then you can order it by calling (800) 922-0204 or take a trip to the nearest Verizon store. • Insert the battery, put on the back cover and then power on the device. • Follow the prompt which the device displays and then it will be activated automatically during the setup process. How to Activate Verizon for HTC, Motorola, LG or any other Android phone • Turn iMessage off if you intend to switch from an iPhone. Go to Settings, then click on Messages, select iMessage and then tap on the slider to turn off the messages. • Call (877) 807-4646 from another phone to activate your 4G LTE plan. This would be unnecessary if your SIM card was already 4G enabled on your previous device. The afore-mentioned number cannot be called from the new Android phone. Follow the directions received from the prompt after calling and remember to keep your purchase bill accessible. • Use a SIM card removal tool to insert your SIM card. Although new HTC phones come with SIM card already installed, but if you happen to activate a used HTC phone; then don’t forget to insert your own SIM card before activation. • Power the phone on by pressing the Power button. Some of them have it at the top, while others like LG G3 have it at the back. • Follow the prompts which appear and you will be guided through the setup process. The activation happens automatically while the setup is functioning. How to Activate Verizon Phone? • Purchase a new Verizon SIM Card: In order to connect to the 4G LTE network, you need to have a Verizon SIM Card. You can order one online via Verizon website or offline via calling Verizon customer care or visiting a Verizon store. • Get IMEI number of Verizon device: Dial *#06# from your phone to get IMEI number of your device. The IMEI number is required at the time of activation. • Activate online: People who already use Verizon Wireless or any other Verizon device have their account online in My Verizon page. These Verizon phone owners simply have to sign in using their user id and password. For others who do not own a Verizon account, simply go through the registration process and get their own My Verizon Page using their main number. After signing in, My Verizon Page will appear. A sidebar will have the option “Activate or Switch Device”, click on it. A new window will open through which they can activate their account. Select to reactivate an old number or to activate a new one. Follow the on-screen commands. The whole process is of 5 minutes duration. • Activate over the phone: You can activate your Verizon phone by dialing *611 from the dial pad. This is a Verizon support number. You can also call (800) 922-0204 or 1-888-294-6804 which are and on prompt, enter your 10 digit phone number. The representative answers the phone; inform him/her that you want to activate your phone number. Some security questions will be asked which you need to answer. He may ask for the IMEI number of device and some proof regarding you being the account holder. After 30 seconds, the Customer representative will ask you to switch off your phone and turn it on. Then dial *228 and send. The phone will be active in 1minute. For any queries, customer care is available from 6am-11pm, 7 days a week. Helpful Verizon codes are: #BAL (#225): Check Your Balance #MIN (#646): Minutes Used #PMT (#768): Make a Payment #DATA (#3282): Check Number for Messages and data usage *611: Customer Service (dial for free from your phone) You can find more verizon number here: • Visit a Verizon Store: Those users who want the technician to activate the phone for them; they can simply visit a Verizon Wireless store and ask them to do the needed. How to Activate Any Non-Verizon phone? • First of all, ensure that the phone you are going to unlocks compatible with Verizon or not. This would save you from a lot of trouble if it wouldn’t work anyway. • Get the IMEI number of your phone by dialing *#06#. Most of the phones would show up the IMEI number from this method, if not then see the mobile’s cover box for it. This number is important as it would be required at the time of activation. • You won’t be able to activate a phone until you unlock it if it has been set up to its original carrier. Most of them would unlock old phones if its contract is over or you can purchase the unlock codes online. • Insert the Verizon SIM card into the unlocked phone. Most phones having a removable battery have SIM slots under the battery while non-removable ones have it on the side. • Call (800) 922-0204. This number is, and the customer service representative would activate the phone for you. You might need to give your IMEI number to prove that you are the original account holder. Attention, Internet Explorer User Announcement: Verizon Wireless Community has discontinued support for Internet Explorer 7 and below. In order to provide the best platform for continued innovation, Verizon Wireless Community no longer supports Internet Explorer 7. Verizon Wireless Community will not function with this version of Internet Explorer. Please consider upgrading to a more recent version of Internet Explorer, or trying another browser such as Firefox, Safari, or Google Chrome. (Please remember to honor your company's IT policies before installing new software!) • • • •. Hi Rajat, Thank you for contacting Microsoft Community. I understand that you have an issue with downloading Bluetooth + Wi-Fi Driver from Dell website. I will surely help you with this issue. This issue could have occurred if the Wireless Switch is turned off in BIOS, I would suggest you to refer the article from Dell Support and check if it helps. ![]() Note: Modifying BIOS/ complementary metal oxide semiconductor (CMOS) settings incorrectly can cause serious problems that may prevent your computer from booting properly. Microsoft cannot guarantee that any problems resulting from the configuring of BIOS/CMOS settings can be solved. Modifications of the settings are at your own risk. If the issue persists, I would suggest you to contact Dell Support for further assistance on this issue. Hope this information helps. If you need any further assistance or information on this issue, reply to this post. I will be glad to help you. How to turn ON bluetooth in Dell Inspiron? Does anybody know any trick to keep the bluetooth ON everytime I switch ON. How enable bluetooth in Dell Inspiron. Dell Inspiron 5520:: Browser Deactivated When Typing And Have To Activate It Again Jan 26, 2013 I bought my Inspiron 5520 in the States in July, 2012. The problem that I have now, which is quite annoying really is that when I type in a browser, it becomes deactivated and I have to activate it again in order to continue typing. It happens very often and it's almost impossible to have typed a long message or a document without a browser being deactivated and me having to activate it again and again. Same happens with Word. Similar Messages: • • • • • • • • • • • • • • • • • • • • • • • • • • • • • ADVERTISEMENT Jan 6, 2013 I'm facing a very strange issue in my Inspiron 15R 5520 which I bought in August 2012. There are few characters, which appear only after typing the corresponding key on the keypad twice. ![]() ![]() If I want to type tilde(~) character, then I have to press the key on the lest side of Numeric key 1. But, if I press it once, nothing appears on the screen. And If I press this key twice, then I get two tilde (~) characters. Same behaviour happens for four other characters. Given below is the list of characters, which do not show up by pressing key once, rather twice. ~ ` ^ ' ' Surprisingly, when I was typing keys Shift+6 in the compose area of this forum, then the character caret (^) appeared after pressing key once. Whereas, if I try it in Notepad or Word Pad or in the address bar of Mozila FireFox, I'll have to press the key twice so that character appears twice and then delete the extra character. Feb 23, 2015 I was playing a game and alt tabbed then i got a blue screen and my computer restarted. After it restarted a blue screen popped up saying activate windows now. What should i do and how do i fix this, because of this my office stopped working as well. Jul 30, 2013 I have Dell Vostro 1400 and I am facing a very wierd issue. My laptop types automatically in the search or in a browser if it is open and in any application that it can type. It mostly types 'asdf;lkj' like this. Again when I use a media player it automatically increase or decreases the volume to the max. And sometimes restart the player. It again sometimes automatically locks my computer. TRIED THINGS: 1. I have already formated my laptop many times and also updated the BIOS as I thought it as a VIRUS problem. Also, I have tried different antivirus software but all in vain. I have also dislodged my laptop keyboard and using an external keyboard; but this is also not working. Prior to all these I have called DELL Service for support. But they also could not offer me a satisfactory answer. Jan 17, 2013 I need to activate the NX processor to install windows 8 but in the bios do not see this option. I have an Inspiron N4050 Dec 21, 2014 I've got a DELL Inspiron 15 3542. With Win7 64 bitsi downloaded all drivers from this sitewhen i try to install Bluetooth driver (Network_Driver_NT9P6_WN_6.30.223.99_A00) it shows an error message 'Activate Bluetooth Using Wireless Switch'. i can't find a solution. Jul 2, 2014 I have this problem of wireless in my 15R 5537. I upgraded from windows 8 to 8.1 recently only to know that wireless driver was incompatible. I then visited the Dell support site and downloaded the wireless driver for the model i'm using and the OS installed. But when I tried to install it I got 'Activate Bluetooth using wireless switch' error message. I didn't know what wireless switch was since (fn+f2) was not working either. I came to know that there is wireless option in bios menu from various posts. But there was none in my laptop under 'Advance' option in bios menu. I run bios version A08. I tried to reinstall old windows 8(with a different CD this time) but wireless didn't work in this one either. Feb 7, 2009 My problem is that when I type quickly, sometimes cursor jumps to different position in text so it spoils what I wrote already before. It's really annoying and I can't find out why is this happening. I thought that maybe it's related somehow to this laptop. Apr 7, 2013 I have an Inspiron 15R SE 7520 and while typing, the cursor frequently jumps around and will begin typing in a previously typed line. This is extremely frustrating. I am running Windows 8 and have looked to find where I can adjust the sensitivity of my touchpad but can't find it anywhere. I've seen other posts for other computers that suggest uninstalling the touchpad driver and reinstalling it. What I should do for my specific laptop to stop this from happening? Do I need to uninstall and reinstall the touchpad driver. If so, how do I do that for the Dell Inspiron 15R SE 7520 running Windows 8? I'm also keeping my hands off the touchpad while typing but the cursor still moves. Dec 26, 2014 I seem to have trouble with my on-screen keyboard when using the Dell Inspiron in tablet mode. Supposedly the on-screen keyboard will pop-up whenever I activate a text-insert area (such as search, or firefox, or IE, or etc) but there is no response. In addition, I should be able to toggle it on/off through 'settings' by swiping right but the keyboard icon is not available in settings when I swipe right. I *can* turn it on by going through. Settings --> Ease of access --> Keyboard on Though technically it shouldn't require those extra steps and I don't know why it isn't popping up on it's own. Also unsure why I can't find the icon in settings. This laptop is brand new so I didn't change anything on it yet. Feb 27, 2013 I recently upgraded my dell inspiron m5110 from windows 7 home premium to windows 8 Professional from the online store at microsoft. I am quite annoyed about my trackpad getting in the way while typing. The cursor will sometimes unclick, jump onto another part of whatever I am typing or click onto everything and erase it! Since i have downloaded windows 8 i have not found a way too disable the trackpad. I use a seperate mouse and i want to permanently disable my track pad. Sep 21, 2003 I have in inspiron 4100 for about 14 months. I have noticed that while typing, the cursor my move back to a different position in the line, move to a previous line, or jump to another text box altogether. It drives me crazy that when I type, I never know if there is some combination of keys or if I accidently touch something to make the cursor change position. Is there by default some combination of keys that cause cursor movement. It has happened 4 times while typing this message. Can this be turned off? Mar 12, 2013 I just got my laptop and having trouble with the keyboard, the top rows are typing double digits, my number keys are having letters in front of them and or symbols appearing in other areas of my page.after two days already tired of backspacing to correct or keep starting over and over again. Apr 7, 2013 how do you disable the touch pad when using a mouse or typing on a Inspiron 15Z Ultrabook? Mar 3, 2013 I have a Dell Inspiron laptop and my mousepad does not work while typing. This is an annoying default setting and it prevents me from being able to play most computer games. I have already tried changing the dell touchpad settings (Control Panel>Hardware and Sound>Dell Touchpad) to no avail, and I am not willing to get a mouse. Mar 5, 2013 I recently downloaded Windows 8 Pro and now the Ctrl F3 shortcut does not work for turning off the touchpad. I have a Inspiron 15R N5110. I need to turn it off while typing. Mar 8, 2013 Dell Inspiron 1764 Laptop keyboard is slow to respond and has typing lag? Feb 19, 2013 I have a dell inspiron N7110 laptop with windows 7 and my cursor keeps jumping back while I am typing. I have unchecked the enhance pointer precision box. Feb 20, 2013 From time to time the cursor jumps to another line whilst I am typing. This can happen on any software, ie MS word, Hotmail. It can even happen when I am entering a user name and password into any system, for example this forum. It is very frustrating and some tiesm a simple email can take a long time to type. Feb 10, 2013 I have an Inspiron 1545 (warranty is expired). Since the first time i used, the cursor moves from place to place while writing. I am using an external keyboard for the moment, but would like to know if it can be fixed. Jan 9, 2010 I own an inspiron 1545 which works properly for the most part, except for a small, but annoying problem. Sometimes, when I type, instead of characters or numbers appearing on the screen, the computer would perform random tasks like try to open a file when I press O (and I am not holding down the Ctrl key), save my work when I press S (again, no Ctrl key), and change my typing language. It used to run on Vista, and was recently upgraded to 7, but the problem persists. For example, typing the word 'frustration' would sometimes lead to this: frustrati (Open dialogue box appears) It's especially annoying when it changes the typing language mid-sentence. Jan 12, 2013 I teach online and my new Dell laptop Inspiron 15 3520 is useless. I cannot use it in online chat with students because now the cursor is moving to other lines of text and inserting my typing. I uninstalled the touchpad driver as that seems to be something recommended here. I tried to reinstall from the Dell website and it failed. So now I not only have an awful computer but a useless one. I'm regretting the Dell decision after just a week. Sep 17, 2009 I have only had this latop 3 months and it is missing keys when I type. It isnt that the laptop is slow and needs to catch up, it just misses letters and I have to keep going back over the text and filling in. It gets very frustrating putting passwords in and letters and numbers miss. Apr 24, 2013 I bought Dell Inspiron 15R 6 months ago. At first, the cursor works well. However, after 2 months usage, the cursor suddenly starts to runaway when I am typing in Microsoft word. Jul 31, 2009 I've browsed the discussions and have seen problems similar to, but not exactly the same as, my problem, hence the new thread. Problem: I have a brand new Inspiron 1545 from Dell online. When I type text in Internet Explorer (e.g. Webmail applications or this forum), even at a relatively slow speed, many of the keys don't seem to register -- that is, letters or spaces whose keys I press do not show up on the screen. As a result, I must type verrry slowly to make sure that every letter/space shows up, or else go back to re-type it. Strangely, I do not have this problem when typing text in, say, Notepad. Feb 16, 2013 I have Dell Inspirion 3520 Laptop, And its come with Window 7 and I have recently its updated with window 8 after this i got a issue that when I start typing the cursor jump to somewhere else or select something. Jan 27, 2013 I have an Inspiron N5050 which has a couple of issues. First issue is the cursor will move to other parts of the screen (where the mouse is) while in the middle typing. The laptop is setup with both Win 7 & 8 and it happens in both Windows versions. How to correct this or should I chuck this up to typical Dell poor quality control?? Jan 11, 2015 A few days ago I was using my laptop when the notification that I had to plug in the charger or my computer battery would die popped up. So I plugged it in and used it for a few minutes when suddenly the screen went black and the computer just shut off while plugged in. I noticed that my charging cord had a green light when it was plugged into the wall but the light went off as soon as it connected to my laptop. I've tried letting it charge, I've tried holding down the power button, I've tried removing the battery and plugging it back in, and I've tried a newer charging cord but the computer still won't turn on. No lights come on even when it is plugged into the wall. Sep 30, 2012 Wireless network is deactivated by hp connection manager after hibernating, sleep and sometimes when I use the computer. I can't see any preferences in HP Connection Manager to correct this, or in Windows power management. What is the solution to keep the wireless network activated ALL THE TIME?? Uinstall of the HP Connection Manager doesn't work, then I can't activate the wireless network again! My computer:HP ProBook 4530s (brand new) Windows 7 Pro (included with the computer) No other power or wireless utilities installed, just HP utilities as installed from the factory. Feb 22, 2013 my first time to own a laptop:D so now i bought this laptop ( inspiron 15r 5520 ) like 4 months ago. Laptop works perfectly fine and i like it:D now though i would like to upgrade it to windows 8 i already got my windows 8 now though im thinking of installing it on my laptop question is this if i were to install it right now what should i do?? Is there any drivers or anything i need to install once more after i install windows 8? Choose the right size for you, from 2GB to Unlimited. No smartphone? Connect your basic phone, tablet, hotspot or connected device to America’s best network. No annual contract. Monthly plans for your smartphone or connected device. Find a plan for your business, no matter how big or small. Connect from abroad or reach out to 200+ countries while in the U.S. Connect tablets, hotspots and other devices on a shared data-only plan. Back up your content, personalize your device and more. Store photos, videos, contacts, music, documents, call logs and text messages. Coverage for damage, loss, theft and Verizon Tech Coach support Estimate your expected monthly payments in two steps. ![]() This guide is for current Verizon Wireless customers who purchased a 4G LTE phone. If you're a new Verizon Wireless customer, refer to the Activating and Setting Up 4G LTE Phone Guide for New Customers. Note: If you're upgrading from iPhone® to a non-Apple phone, you should turn off iMessage® before activating. You're just minutes from enjoying your Verizon service. You confirm you are the authorized account holder. Connect to your new home Wi-Fi network. To activate a device: • Go to the page in My Verizon. • Tap Activate a Device on an Existing Line. • If you have multiple lines on your account, select the line you want to activate the device on. • In the Check Device step, we'll make sure the device you're activating is compatible with the plan and features on the line. If you've previously used this device on your account, select Yes and pick this device from the drop down. If this is the first time using this device on your account, select No. Enter your Device ID and tap Check Device. To learn how to find the Device ID for your phone, choose your device type and brand from the dropdown menus. • For the security of your Verizon account, complete the authorization process by providing your unique authorization code. We'll send the code to you in a text message. To complete the online authorization process: • Select which phone on your account you want the code sent to. • Tap Text Me the Code. • Enter the code from the text message into the Online Authorization Code field and tap Confirm. • In the Check SIM step we'll make sure your SIM card is compatible with your line. If your device doesn't require a SIM card or the previously used SIM card is compatible, you'll skip this step. Enter your SIM ID number and tap Check ID. To learn how to find your SIM ID number, tap Finding Your SIM Card and SIM ID. If you don't have a SIM card, tap Add to Cart to order one. • Select an equipment protection option and tap Confirm. You’ll only see this step if you previously had equipment protection or have recently upgraded. • If the device you're activating is not compatible with your current plan, you'll be asked to select a compatible plan. Select the plan you want and tap Confirm. • Review your changes and tap Confirm Feature & Plan Changes. • Back up your contacts and media from your old device. You've successfully activated the device. You can also watch our for instructions. Notes: • These are instructions just for My Verizon. If you'd like more detailed activation instructions, enter 'activate [device name]' (e.g., 'activate G3') in the Search field on our website. Some of the first search results should be activation guides for the device title you entered. • Visit our to learn how to activate a prepaid device. • If you'd like to get a new device, refer to our. • If you have problems activating a device, call Customer Service at (800) 922-0204. You can switch devices between two lines on your account if you're the. To switch devices between two lines on an account: • Go to the page in My Verizon. ![]() • Tap Switch Active Devices. • Select the two devices you want to switch and tap Next. • If the two lines have different equipment protection options, they may not be compatible with the devices you're switching. If so, you'll be asked to update each line with a compatible equipment protection option. To update equipment protection: • Select the equipment protection option you want. • Tap Confirm. • If the two lines have different plans, they may not be compatible with the devices you're switching. If so, you'll be asked to update each line with a compatible plan. To update your plan: • Select the plan option(s) you want. • Tap Confirm. • Review your changes and tap Confirm Feature & Plan Changes. • Backup your contacts and media from your old device. • Reset both of your devices and turn them off. Tap Devices are off. • Tap Swap Devices. • Tap Yes to confirm both devices are off and you are ready to swap them. You've successfully switched devices between two lines on your account. Watch our to learn more. You'll need to back up your contacts and other information on your original device. You can do this using one of the following methods: • Verizon Cloud - Use this method if your original and new devices are both. • - Use this method if your original or new device isn't compatible with Verizon Cloud. • - Use this method if your original or new device isn't compatible with either option listed above. Before transferring your content, you'll need to you're going to start using, and then you can transfer your content from your original device to your new device. Learn how to transfer content if you're: • •. If you currently have a on your account, you won't be able to activate a new device online until the pending order is completed or removed from your account. Learn more on our page. An or can remove a pending order from an account online in My Verizon.. Note: If a pending order is removed, then it's canceled and the order will have to be placed again. To remove a pending order from your account: • Go to the page in My Verizon. • Click View pending account changes. • Click Delete next to the pending order you wish to remove. • Click Delete pending account change. Choose the right size for you, from 2GB to Unlimited. No smartphone? Connect your basic phone, tablet, hotspot or connected device to America’s best network. No annual contract. Monthly plans for your smartphone or connected device. Find a plan for your business, no matter how big or small. Connect from abroad or reach out to 200+ countries while in the U.S. Connect tablets, hotspots and other devices on a shared data-only plan. Back up your content, personalize your device and more. Store photos, videos, contacts, music, documents, call logs and text messages. Coverage for damage, loss, theft and Verizon Tech Coach support Estimate your expected monthly payments in two steps. Follow these instructions to activate your new or Certified Pre-Owned 4G LTE smartphone. Note: If you're upgrading from iPhone速 to a non-Apple phone, you should before activating your new phone. Step 1: Save contacts from your old device. Note: If you are new to Verizon Wireless, cannot access your old device or have already backed up your contacts using Backup Assistant, go to step 2. Contacts and media will not automatically transfer to your new device. You will need to back them up so you can restore them later on your new device. • Back up contacts Refer to the for instructions on backing up your contacts. • Back up media You can back up pictures, videos and music by saving them to a memory card or with 1. Refer to the V CAST Media Manager website for more information on using this service. Step 2: Activate your 4G LTE SIM card. Notes: • If you are using your existing Verizon Wireless 4G LTE SIM card, skip to step 3. • If you are a business customer and this is your first 4G LTE device, your 4G LTE SIM card is preinstalled on your new Smartphone, skip to step 4. To activate your SIM card, dial (877) 807-4646 from any phone and follow the prompts to activate your 4G LTE service. Have your Customer Receipt with you because you may be asked to confirm information from it, including the mobile number assigned to your 4G LTE SIM card. Step 3: Insert your SIM card and battery into your 4G LTE Smartphone. • If you received a 4G LTE SIM card in your shipment, snap the SIM card out of its holder without touching the gold electrical contacts. Or If you are using your existing Verizon Wireless 4G LTE SIM card, remove the SIM card from your old device without touching the gold electrical contacts. Note: The actual insertion process varies by device, so refer to your user guide for instructions on how to insert the SIM card. You can find your user guide by going to the and clicking on User Guide. • Insert the battery and battery cover. • Charge your new device. Step 4: Activate your new 4G LTE Smartphone and transfer your contacts. • Turn on your new 4G LTE device; activation will begin automatically. • Follow the on-screen prompts to set up your device and download or activate Backup Assistant 2 to transfer your contacts. Refer to the for instructions on backing up your contacts • If you back up media from your old device, restore it to your new device using one of the following methods: • If you backed up your media to a memory card, insert the card into your new device. • If you backed up your media using V CAST Media Manager, access the application and follow the prompts for restoring. Refer to the V CAST Media Manager website for more information on using this service. 1 Only available to consumer customers with compatible devices who are enrolled in My Verizon. Or charges, as applicable, will apply for all downloads. Does not apply to business customers, federal / state government customers or Smartphone users. 2 For prepaid customers, data charges may apply for download (1 MB) and usage. Compatible device required. Apple, iPhone and iMessage are registered trademarks of Apple Inc. ![]() ![]() Previously, we have shown transcriptional activation of endogenous genes in plants using dCas9-VP64. Here, we develop a second generation of vector systems for enhanced transcriptional activation in plants. We tested multiple strategies for CRISPR-Cas9 based transcriptional activation. Simultaneous recruitment of VP64 by dCas9 and a modified guide RNA scaffold gRNA2.0 (designated CRISPR-Act2.0) yielded stronger transcriptional activation than our first generation dCas9-VP64 activators. In addition, we have built a multiplex transcription activator-like effector activation (mTALE-Act) system for simultaneous activation of up to four genes in plants. Galweiler, D. Grosskopf, J. Schell and K. Palme (1998) A transcription activation system for regulated gene expression in transgenic plants. Proceedings of the National Academy of Sciences of the United States of America 95, 376–381. Ouwerkerk, R.J. De Kam, J.H.C. Hodge and A.H. Meijer (2001). Jan 05, 1998 A transcription activation system for regulated gene expression in transgenic plants. Transcriptional Activation*. Our results suggest that mTALE-Act is even more effective than CRISPR-Act2.0. Moreover, we explored tissue specific gene activation using positive feedback loops. Interestingly, our study revealed that certain endogenous genes are more amenable than others to transcriptional activation, and tightly regulated genes may cause target gene silencing when perturbed by activation probes. Hence, these new tools may be used to investigate gene regulatory networks and their control mechanisms. Assembly of multiplex CRISPR-Act2.0 and mTALE-Act systems are both based on streamlined and PCR-independent Golden Gate and Gateway cloning strategies. The systems will enable transcriptional activation applications in both dicots and monocots, and the vectors in this new toolbox are publicly available to the research community through Addgene. A widely applicable promoter system is described that allows a gene of interest to be activated in specific plant tissues after a cross between defined transgenic lines. The promoter, pOp, consists of lac operators cloned upstream of a minimal promoter. No expression was detected from this promoter when placed upstream of a β-glucuronidase (GUS) reporter gene in transgenic plants. Transcription from the promoter was activated by crossing reporter plants with activator lines that expressed a chimeric transcription factor, LhG4. This factor comprised transcription-activation domain-II from Gal4 of Saccharomyces cerevisiae fused to a mutant lac-repressor that binds its operator with increased affinity. When LhG4 was expressed from the CaMV 35S promoter, the spatial and quantitative expression characteristics of the 35S promoter were exhibited by the GUS reporter. The LhG4/pOp system may be used to study toxic or deleterious gene products, to coordinate the expression of multiple gene products, to restrict transgene phenotypes to the F1 generation, and to generate hybrid seed. The LhG4 system offers spatially regulated gene expression in the tissues of whole plants growing under normal conditions without the need for external intervention. It complements inducible expression systems that offer temporal control of gene expression in tissues that can be treated with inducing chemicals. Plant science, both basic and applied, relies increasingly on the use of transgenes to manipulate biological processes in transgenic plants. Thus antisense RNA, dominant negative mutants, dominant gain-of-function mutants, ectopic expression, and overexpression have provided powerful tools to investigate a broad range of biochemical pathways. However, some of these molecular genetic approaches are not easily applied to genes that control fundamental processes of plant growth, differentiation, and reproduction. This is primarily because manipulating such critical genes can be severely detrimental to plant growth and survival and so preclude the generation and propagation of useful transgenic plants. One solution has been to use “tissue-specific” promoters to restrict the activity of transgenes to certain tissues (, ). This approach is far from satisfactory as many such promoters are active during the process of regenerating transgenic plants. Tissue specific expression also restricts the scope of the analysis to a few cell types and does not always allow the appropriate tissues to be studied. Another option is the use of heat-shock promoters that can achieve relatively high level expression in many cell types (). This has the disadvantage that the subsequent analysis is performed on plants subjected to heat stress, growth conditions must be carefully controlled, and problems have been encountered with expression of genes in plants not induced by heat shock (–). To overcome these limitations several attempts have been made to develop chemically regulated gene expression systems (–). ![]() Again, these are not entirely satisfactory as they are either relatively inefficient (high background and/or only modest induction), dependent on sustained gene repression, not applicable to commonly studied plant species, or reliant on the application of chemicals at concentrations that may be toxic to plants. The best characterized system is a tetracycline inducible promoter developed for tobacco (, –). This chemical induction system provides useful temporal control of gene expression in tobacco cells though its usefulness and reliability for spatial control of gene expression in the various tissues of whole plants is less clear. Also it appears that this system is not effective in Arabidopsis (). It is necessary to establish a generally applicable system for effective spatial control of transgene expression that does not require external intervention or imposition of environmental stress. Here we report a system based on chimeric transcription activators and promoters. In essence, “reporter plants” are generated with the gene of interest cloned downstream of a promoter that comprises simply a TATA-box and binding sites for a transcription factor that is not present in plants (Fig. Consequently, the gene of interest is unlikely to be expressed when introduced into wild-type plant cells. One simple way to regulate expression of a transgene is to equip it with a known promoter that is sensitive to a physiological signal. All involve the drug-dependent recruitment of a transcriptional activation domain to a basal promoter driving the gene of interest, but. This allows phenotypically normal transgenic lines to be generated and propagated without interference from the transgene or selection against its expression. Expression of the transgene is achieved by crossing these reporter plants with an “activator plant” that expresses a novel transcription factor that specifically recognizes the binding sites in the transgene promoter (Fig. In this way the gene of interest will be expressed only in those cells of the progeny in which the transcription factor is expressed, and this will depend in turn on the promoter selected to express the transcription factor. Thus the effects of the transgene can be studied in the progeny of crosses between activator and reporter lines. RESULTS To construct a stringent regulatory system based on promoter activation we sought to use transcription factors with sequence-specific DNA-binding activities that were not normally found in plants. Initially we selected the Saccharomyces cerevisiae Gal4 protein that had been used successfully in an analogous regulatory system for Drosophila () and shown to act as a transcription factor in transient expression experiments with tobacco (). Subsequently we developed an alternative system based on a modified Escherichia coli lac-repressor. Here we describe only the lac-repressor system as this appeared to be the more effective. The details of our initial Gal4-based system will be presented elsewhere. Chimeric Transcription Activators Based on the lac Repressor. Many eukaryotic transcription activators have an apparently modular organization with separable domains for DNA-binding and for interaction with the transcription apparatus (–). ![]() A fusion between the E. Coli lac repressor and the VP16 activation domain had been shown to be a potent transcriptional activator in mammalian cells (). Lac repressor expressed in plants can apparently also bind to plant chromatin (), so we attempted to convert lac repressor into an effective transcriptional activator for plants. The chimeric transcription activator, LhG4, was based on a lac repressor mutant, lacI his, encoding a repressor in which tyrosine 17 in DNA-binding-helix-2 is replaced by histidine. The lacI his protein was estimated to bind lac operator sequences with at least 100-fold greater affinity than wild-type lac repressor (). A sequence encoding Gal4 transcription-activation-domain-II (residues 768–881, 17) was fused in frame with lacI his at codon 329, near its C terminus. This fusion, LhG4, was inserted between a CaMV 35S promoter and polyadenylation signal in a binary Ti-plasmid vector to generate pLh-G4 (Fig. For comparison, a lac repressor derivative, LacI-VP16, that had been optimized for use as a transcription activator in mammals (), was cloned in the same binary vector, to generate pL-VP16. This construct differed considerably from pLh-G4 as it had a wild-type lac repressor DNA-binding domain, a simian virus 40 large T-antigen nuclear-targeting signal, and the herpes simplex virus VP16 activation domain. PLh-0, which expresses the lacI his coding sequence without a transcription activation domain served as a negative control. To construct the reporter plasmid pOp-GUS, a glucuronidase (GUS) reporter gene was cloned downstream of a minimal CaMV 35S promoter (+8 to −50 relative to the transcription start site) and two copies of an optimized lac operator were inserted upstream (operator 310 of ref. To reduce the risk of the minimal CaMV 35S promoter being activated in cis by promoter sequences in the T-DNA or neighboring plant DNA, the minimal promoter in pOp-GUS was placed at least 2kb from the left and right T-DNA border sequences, and 2.5 kb away from the only fully functional plant promoter on the T-DNA, the relatively weak () nopaline synthase promoter. Plasmid pTA-GUS was identical to pOp-GUS except that it lacked the operator sequences. LhG4 Is Functional in Plants and Their Progeny. Transient expression experiments in tobacco protoplasts with pLh-G4 (activator), pOp-GUS (reporter), and the control constructs provided initial evidence for activation of reporter gene expression by LhG4 (data not shown). This activation was found to be dependent on the presence of both lac operator sequences in the reporter construct and the Gal4 activation-domain in the activator construct, so we proceeded to test activator and reporter constructs in stable transformants. Leaf samples from 10 independent rooted primary transformants generated with reporter plasmid pOp-GUS were screened for GUS activity using the histochemical assay, and none showed detectable activity even in prolonged (16 hr) assays. This confirmed that the GUS reporter construct was not efficiently activated in cis from the T-DNA or flanking chromosomal DNA in these plants and that, as expected, tobacco cells do not have a transcriptional activator that can recognize the lac operator sequences used here. Six pOp-GUS transformants (16/1, 16/3, 16/12, 16/15, 16/51, and 16/56) were selected as reporter plants for retransformation with the activator plasmids pLh-G4 and pL-VP16. Retransformants, selected by their resistance to methotrexate, were screened for GUS activity by histochemical assay of leaf pieces. Of 40 independent reporter plants retransformed with pLh-G4, 10 showed intense staining similar to or stronger than 35S-GUS plants, two exhibited intermediate staining and two more showed weak staining. The distribution of GUS-expressing retransformants among the individual reporter plants appeared to be nonrandom. Thus strong or intermediate staining was observed in 5 of 12 retransformants derived from reporter 16/12 and in 7 of 8 retransformants derived from reporter 16/56, but not in any of the 20 retransformants regenerated from the remaining four reporter plants (16/1, 16/3, 16/15, and 16/51). This probably reflects the influence of the integration site and flanking plant DNA on the expression of the reporter gene in each reporter line, and suggests that in a favorable location the reporter may be effectively activated by most activator loci. Of 19 independent reporter plants retransformed with pL-VP16 (which encodes LacI-VP16), one showed intermediate staining and a further five showed weak staining, suggesting that pL-VP16 is a less effective activator construct than pLh-G4. This suggestion was supported by analysis of GUS transcript abundance in 16/56 retransformants (Fig. Five pLh-G4 retransformants that had shown strong GUS activity were all found to express both GUS and LhG4 transcripts of the expected size. In a sixth retransformant, line 16/56-G3 in which no GUS staining or transcript was observed, the only detectable LhG4-homologous transcript was much larger than expected. In marked contrast to the pLh-G4 retransformants, GUS transcripts were barely detectable or undetectable in any of the pL-VP16 retransformants, even though LacI-VP16 activator transcripts were generally far more abundant than LhG4 transcripts. As LacI-VP16 differs in several respects from LhG4 (see above) there are a number of possible explanations for its apparently poorer activity and greater transcript abundance. At this stage we conclude only that pL-VP16 is a less effective activator construct than pLh-G4. RNA gel blot analysis of reporter line 16/56 retransformed with pLh-G4 and pL-VP16 activator constructs. RNA was isolated from reporter line 16/56 (R-16/56) containing pOp-GUS alone and from several lines retransformed with the. The cauliflower mosaic virus (CaMV) 35S-promoter is reported to be more active in the older leaves than in younger ones (). To test whether this is reflected by the GUS reporter when its expression is directed by LhG4 under CaMV 35S control, extractable GUS activity was measured in 6 successive leaves on each of 5 pLh-G4 retransformants derived from reporter plant 16/12. There was a progressive increase in expression levels from an average of 3.2 nmol 4-methyl-umbeliferone (MU)⋅min −1⋅mg −1 in the young leaves to an average of 17.4 nmol 4-MU⋅min −1⋅mg −1 in the older leaves (data not shown). The extractable GUS activities in the older leaves of these plants are similar to those of tobacco plants carrying CaMV 35S GUS constructs (typically 10–30 nmol⋅min −1⋅mg −1 ()). When GUS expression in these retransformed reporter plants was assayed over a period of several months it was found to be either consistently high or consistently absent in individual lines with the exception of line 16/12-G3 that occasionally failed to show GUS expression. In this plant GUS activities were found to vary from. Reporter expression in the progeny of plants transformed sequentially with reporter construct pOp-GUS and activator construct pLh-G4. Transgenic plants derived from reporter lines 16/12 and 16/56 by retransformation with pLh-G4 were allowed. Extractable GUS activity measured in T2 seedlings derived from the 16/12 and 16/56 reporter plants was indistinguishable from that of untransformed plants (. The GUS Reporter Is Expressed After Crossing Activator and Reporter Plants. The aim of this work was to generate a system that would allow gene expression to be activated by crossing reporter and activator plants. To test whether this was possible with the pOp/LhG4 system, activator plants were generated by transforming tobacco with pLh-G4. Three such activator plants (10/1–2, 10/1–6, and 47/1–21) were chosen at random and each was crossed with the reporter plants 16/12 and 16/56. The same three activator plants were also crossed with a control plant, 13/4, that had been transformed with pTA-GUS that contains a GUS reporter gene driven by the minimal CaMV 35S-promoter but without lac operators upstream. The frequencies of hygromycin and methotrexate resistant F1 seedlings suggested that each activator plant contained a T-DNA at a single locus and confirmed that the reporter plants had T-DNAs at 4 or more loci. F1 seedlings resistant to both hygromycin and methotrexate were assayed histochemically for GUS activity. This demonstrated that all three randomly selected activators were able to induce GUS expression in the F1 progeny of both reporter plants but not in the progeny of the negative control plant 13/4 (Fig. Activator plant 10/1–6 was able to activate GUS expression to 35S-GUS levels in all seedling organs after crossing with either reporter plant. In contrast, GUS expression induced by activator 47/1–21 was weaker and was restricted principally to cotyledons and hypocotyls. In subsequent experiments, the GUS reporter construct was found also to be expressed as expected in the F2 progeny of 10 randomly selected F1 plants derived from a cross between reporter 16/56 and activator 10/1–6 (data not shown). Thus the pOp/LhG4 system was active in three successive generations of transgenic plants. DISCUSSION We have established a promoter and transcription factor system that allows a gene of interest to be expressed only after crossing of reporter and activator plants. This system may be useful ( i) if the gene of interest is expected to interfere with regeneration or propagation of transgenic material, ( ii) if its activity is required only in the progeny of a cross, or ( iii) if it must be expressed in several different specific tissues. It may also allow the expression of several transgenes to be regulated coordinately without multiplication of plant promoter sequences. The promoter comprises a minimal promoter fragment of the CaMV 35S-promoter with two ideal () lac operator sequences cloned upstream. The transcription factor, LhG4, consists of a high affinity DNA-binding mutant of the E. Coli lac repressor () fused to a transcription activation domain from the yeast Gal4 protein. We envisage that this promoter may be used to introduce a gene of interest into tobacco where it will initially be silent. In this way transgenic “reporter” plants may be regenerated without interference from the transgene. This would be advantageous when the gene of interest is expected to interfere with some essential metabolic or cellular function or when its activity is required in the F1 generation only. The use of constitutive promoters in such cases is likely to lead to the recovery of plants in which transgene expression is weak and variable owing to position effects and gene silencing phenomena. In the pOp/LhG4system described here, the gene-of-interest can be effectively activated by crossing reporter plants with plants that express LhG4, allowing the full range of transgene expression to be achieved in the progeny. If this prevents the progeny from being propagated, more experimental material can be generated by crossing the phenotypically normal activator and reporter plants. When GUS was used as the reporter in this system, enzyme activity increased from 10% repressor binding activity is unlikely to occur more than once in 10 10 bases; however it remains to be determined how effectively and over what range LhG4 can activate gene expression from these sub-optimal sequences. Nevertheless, the activator plants obtained so far appeared normal and transmitted the activator T-DNA as a simple Mendelian locus. A number of alternative strategies have been reported for regulation of transgene expression in plants. The most well characterized are a soybean heat-shock promoter (, ), and three chemically inducible gene expression systems (,, ). Use of the heat-shock promoter requires that growth conditions are permanently under stringent control to avoid stressing the plants unintentionally. Furthermore, experiments can be performed only on heat-stressed material, and the heat-shock promoter offers little opportunity for spatial control of gene expression. The best characterized chemically-inducible expression system is the tobacco tetracycline system (, –) that offers effective temporal control of gene expression. However it is not clear whether all tissues and organs are accessible to the inducer and receive similar doses, there is little spatial or tissue-specific control of gene expression, the inducer must be repeatedly applied to ensure continued transgene expression, and it has proven difficult to establish the system in other species (). Recently, a promising steroid-inducible expression system has been established by replacing the DNA-binding and transcription-activation domains of the rat glucocorticoid receptor with those of Gal4 and VP16 respectively (). However the long-term toxicity of the inducing steroids and the efficiency of reporter expression in the various organs of mature plants growing under normal physiological conditions have not yet been established. The pOp/LhG4 expression system is complementary to the chemical induction systems described above and appears to offer three distinct advantages. Firstly transgene expression can be induced under normal environmental and physiological conditions without external intervention. Secondly high levels of reporter expression can be achieved and sustained reliably, even in tissues that might be inaccessible to inducing chemicals, without the need to continually apply an inducer. Finally spatial control of reporter expression may be achieved by use of appropriate promoters to express LhG4. Weinmann et al. () reported a chimeric plant transcription factor system based on a tet-repressor-VP16 fusion. Reporter expression was efficient in the primary transgenics and was sensitive to tetracycline, but it became considerably weaker as the plants matured and these weaker expression levels were sustained in subsequent generations (). Alternative tRNA-based regulatory systems (, ) provide stringent repression of transgene function in the absence of a suppressor tRNA. However, they require specific mutagenesis of the gene-of-interest to introduce a stop codon, achieve only low level synthesis of translation product, and almost certainly interfere with the translation of endogenous mRNAs. Nonetheless, these may be the systems of choice when the transgene product is active at low concentrations and stringent repression is paramount. In summary, we have developed a system that appears to offer stringent, noninvasive, spatial control of plant gene expression. In future we aim to generate a series of activator lines with LhG4 expressed in various specific tissues. We also envisage that a chemically inducible and spatially controlled expression system may be obtained by bringing LhG4 under steroid hormone control through fusion to the ligand-binding domain of the rat glucocorticoid receptor (,, ). EXPERIMENTAL PROCEDURES DNA cloning, manipulation, and analyses were all performed according to standard methods (). Agrobacterium tumefaciens strains were GV3101∷pMP90 and GV3101∷pMP90RK. Plant material was Nicotiana tabacum cv. Petit Havana SR1 (). Transformation of tobacco was achieved by the leaf-disc method. Hygromycin, kanamycin, and methotrexate were added to culture media at 20 μg/ml, 50 μg/ml, and 0.5 μg/ml, respectively. GUS activity was determined and histochemically localized according to Jefferson (). Transient expression experiments were performed with tobacco mesophyl protoplasts as described in ref.. RNA Gel Blot Analysis. Total RNA was isolated from plant tissues based on the procedure of Chomczynski and Sacchi (), and was dissolved in formamide. Equal concentrations were electrophoresed and blotted according to ref.. To detect GUS transcripts, a 1.8kb BamHI- SacI fragment containing the GUS coding region () was used. To detect LhG4 and LacI-VP16 transcripts, an XhoI- XbaI fragment was isolated from pKIHisA0 (see above). This fragment was chosen as it hybridizes with equal efficiency to LhG4 and LacI-VP16 transcripts. I use Cmder on windows 10 with Python 3.5 and virtualenv on my laptop and work computer just fine. Now I have a new computer running Windows 10, Python 3.5, virtualenv 15.0 and I am able to initialize a python virtual environment with: virtualenv env and I get the virtualenv directories but when I run: env/Scripts/activate Nothing happens. No error message and worst of all nothing to signify that the environment was activated or not (i.e. The string '(env)' does not pop up in the terminal next to the path). The odd thing is that I can activate a venv when using plain old cmd and the '(env)' shows up in the terminal. Just not when run in Cmder. Another oddity (which I don't think is related but may be) is that once I can get a virtualenv running in regular cmd, it says the 'deactivate' keyword is not recognized when I try to shut it off. I found this post (below) on StackOverflow which is similar but not all that useful as it doesn't actually have an answer and is more about PowerShell. Any thoughts? ![]() Virtual Python Environment builder. Virtualenv is a tool to create isolated Python environments. The basic problem being addressed is one of. ![]() ![]() ![]() ![]() ![]() Kaspersky 2017 products — Kaspersky Internet Security 2017, Kaspersky Total Security 2017 and Kasperksy Antivirus 2016— have been released and available for purchase or free for testing in the form of trial version, for 30 days. 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